KT12 human cell line

IHW Number: 9073, 88012503

• Sinonimo/i: LKT12
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human blood
• Confezionamento: tube of 5 μg 88012503-DNA-5UG; pkg of vial of cells 88012503-1VL
• Etnia: Japanese
• Genere: male
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Consanguineous: No

Descrizione della linea cellulare

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profilo DNA

A: 2402 31012
Cw: 0401 1202
B: 3501 5101 (!)52
HLA DRB1: 09
HLA DQB1: 03
HLA DPB1: 0402 0501

Profilo sierologico

HLA A: 24 31
HLA A ief: 24.1 31
HLA C: 4
HLA B: 35 51
HLA B ief: 35.3 51
Bw4/bw6: 4 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 5

Terreno di coltura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Mantenimento delle subcolture

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Altre note

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Esclusione di responsabilità

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.