Grunt Fin (GF)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: fish fin
• Confezionamento: tube of 5 μg 88010601-DNA-5UG; pkg of vial of cells 88010601-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 47
• Morfologia: Fibroblast-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Fish Grunt fin tissue blue striped

Descrizione della linea cellulare

Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.

Terreno di coltura

EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO₃ + 20% FBS (Heat Inactivated)+ 58mM NaCl

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.