6-23 (clone 6)

87042206, rat thyroid, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: 6-23 (clone 6), 87042206
• Origine biologica: rat thyroid
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Neurotensin and calcitonin
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat medullary thyroid carcinoma

Descrizione della linea cellulare

Derived from the non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.

Applicazioni

Hormone studies

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells need to be grown on flasks coated with matrigel or any other attachment factor

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. For cell attachment culture vessels need to be coated with collagen type IV e.g. Sigma product C-5533. Soak in a sol

Altre note

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