Detroit 551

86082802, human skin (embryonic), Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: Detroit 551, 86082802
• Origine biologica: human skin (embryonic)
• Confezionamento: tube of 5 μg 86082802-DNA-5UG; pkg of vial of cells 86082802-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 46
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian embryo skin

Descrizione della linea cellulare

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

Applicazioni

Chromosome and virus studies (Normal control): herpesviruses, SV40

Profilo DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.