AFRC MAC 77

85102262, Reactivity: Anti-Nitrate Reductase

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Rat x Rat Hybridoma
• ImmunoDon: F344 rat spleen
• Mieloma: Y3Ag1.2.3
• Reattività: Anti-Nitrate Reductase
• tecniche: cell culture | mammalian: suitable
• Isotipo: IgG2b
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat Y3Ag1.2.3 x Rat F344 spleen

Descrizione della linea cellulare

The antibody specific for NADH nitrate reductase and inhibits the reduction of nitrate to nitrite using NADH as electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) and methyl viologen. The antibody is directed against NADH-NR of mono and dicotyledons.

Immunogeno

Nitrate reductase purified from the leaves of 6 to 8 week old spinach plants - Spinacea oleracea l

Terreno di coltura

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Altre note

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