Do-11-10

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85082301, mouse unknown/unspecified, Not specified

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse unknown/unspecified
• Confezionamento: tube of 5 μg 85082301-DNA-5UG; pkg of vial of cells 85082301-1VL
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Not specified
• Recettori: Not specified
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

Descrizione della linea cellulare

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

Terreno di coltura

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

Altre note

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