MM138 Cell Line human

10092321

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human skin
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human melanoma; from metastatic site - lymph node

Descrizione della linea cellulare

Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.

Terreno di coltura

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).

Mantenimento delle subcolture

Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x10⁴ cells/cm²). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Altre note

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.