N1E-115-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 8062511, mouse nerve, neuronal

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse nerve
• Confezionamento: tube of 5 μg 08062511-DNA-5UG; pkg of vial of cells 08062511-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: modal no. of chromosomes is 192 see reference
• Morfologia: neuronal
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse neuroblastoma

Descrizione della linea cellulare

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Applicazioni

can be used as a neuronal model

Terreno di coltura

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Altre note

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