SAF-1
122301, fish fin, Fibroblast-like
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About This Item
Codice UNSPSC:
41106514
Prodotti consigliati
Nome del prodotto
SAF-1, 00122301
Origine biologica
fish fin
Confezionamento
tube of 5 μg 00122301-DNA-5UG
pkg of vial of cells 00122301-1VL
Modalità di accrescimento
Adherent
Cariotipo
Not specified
Morfologia
Fibroblast-like
Prodotti
Not specified
Recettori
Not specified
tecniche
cell culture | mammalian: suitable
Condizioni di spedizione
dry ice
Temperatura di conservazione
−196°C
Origine della linea cellulare
Gilt head seabream caudal fin
Descrizione della linea cellulare
SAF-1 has been developed from the fin tissues of an adult gilt-head seabream (sparius aurata) without immortalising treatments. SAF-1 supports the replication of several common fish viruses such as lymphocystis disease virus (LDV), infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicaemia virus (VHSV). SAF-1 has a higher susceptibility to rhabdoviruses, especially UHNV. In the SAF-1 line, neither chromosome changes or differences from passage 50-70 have been detected, suggesting a normal karyotype and stable cell line. The cell line may be useful in isolating and studying undetected latent and potential lytic viruses infecting gilt-head seabream and other related sparid species. In addition to its proven applications, SAF-1 could be used as an in vitro system to address multiple questions related to the genetic engineering of this species, such as evaluating the efficiency of the promotors in transgenic constructions, testing whether this species is susceptible to viral vectors available in fish or whether heterologous transposable elements can be mobilised for insertional mutagenesis. This cell line was mycoplasma eradicated at ECACC.
Applicazioni
Genetic engineering, virus susceptibility, fish physiology.
Terreno di coltura
Mantenimento delle subcolture
Split sub-confluent cultures (70-80%) 1:3 -1.6 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin/EDTA; 25°C; no CO2.
Altre note
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