CB0500

CelLytic^™ B Plus Kit

For bacterial lysis

• Sinonimo/i: Lysis and protein extraction kit
• Numero CE: 200-664-3
• Codice UNSPSC: 41106511
• NACRES: NA.56

Proprietà

• Livello qualitativo: 200
• impiego: kit sufficient for 50 g fresh or frozen cell paste lysis.
• tecniche: protein extraction: suitable
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The CelLytic^™ B Plus Kit is designed to efficiently lyse cells and extract proteins from both Gram negative, and difficult to lyse, Gram positive bacteria. This is accomplished using the standard CelLytic B, a proprietary non-ionic detergent in concert with lysozyme, Benzonase^®, and protease inhibitors. This complete kit takes the guesswork out of protein extraction from a variety of bacterial species.

Applicazioni

This kit is compatible with FLAG^®, HIS-Select^®, and glutathione S-transferase based affinity chroma­tography protein purification systems. The detergent included in the CelLytic B Lysis Reagent can be removed from the protein by dialysis or ammonium sulfate precipitation, if necessary. The final purity of the recombinant product is usually higher than that obtained from traditional extraction methods. This is because the crude protein solution containing the non-ionic detergent eliminates much of the non-specific binding that occurs during chromatography. The mild detergent does not interfere with many enzyme assays or protein assays.

Caratteristiche e vantaggi

• Lyse Gram positive and Gram negative bacteria
• More efficient than sonication
• Compatible with affinity purification
• Isolate inclusion bodies for subsequent solubilization
• Non-denaturing cell lysis preserves protein function

Altre note

The CelLytic^™ B Plus Kit contains all of the reagents and chemicals necessary to lyse both Gram-negative and Gram-positive bacteria. This kit also includes protease inhibitors to help prevent the proteolytic breakdown of proteins.

Note legali

Benzonase^® Nuclease is manufactured by Merck KGgA, Darmstadt, Germany and its affiliates. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany.

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

CelLytic is a trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

• B7435 CelLytic^™ B Cell Lysis Reagent, For bacterial cell lysis, standard strength
• E1014 Benzonase^® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
• P8849 Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
• L3790 Lysozyme from chicken egg white, 10 mg/mL

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H315 - H319 - H334
• Consigli di prudenza: P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable