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Key Documents

C8104

Sigma-Aldrich

Monoclonal Anti-Ceramide antibody produced in mouse

clone MID 15B4, purified immunoglobulin, buffered aqueous solution

Sinonimo/i:

Ceramide Antibody, Ceramide Antibody - Monoclonal Anti-Ceramide antibody produced in mouse

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

MID 15B4, monoclonal

Forma fisica

buffered aqueous solution

tecniche

immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:10

Isotipo

IgM

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is produced in response to cellular stimulation by hormones, cytokines and antigens. Ceramide synthesis is mediated either by the salvage pathway by the acylation of sphingosine or sphingolipids hydrolysis or by de novo pathway via dihydroceramide formation. Structurally, ceramides have a sphingoid base, a long-chain amino alcohol linked to fatty acid by an amide bond. Ceramide is generated by hydrolysis of sphingomyelin by sphingomyelinase.

Specificità

Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.

Immunogeno

ceramide conjugated to bovine serum albumin.

Applicazioni

Monoclonal Anti-Ceramide antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunolabeling in electron microscopy
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • as a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.

Azioni biochim/fisiol

Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. They are directly involved in the proliferation and differentiation of skin cells and regulate skin barrier functionality. Ceramide based analogs are potential anti-tumor agents and are regarded as tumor suppressor lipid. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.

Stato fisico

Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.

Nota sulla preparazione

Purified from ascites fluid by gel filtration using sephacryl S-300 resin.

Risultati analitici

The antibody was characterized on ceramide covalently bound to myoglobin.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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ELOVL4-mediated production of very long-chain ceramides stabilizes tight junctions and prevents diabetes-induced retinal vascular permeability
Kady NM, et al.
Diabetes, 67(4), 769-781 (2018)
Ignazio Restivo et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Cell death program of red blood cells (RBCs), called eryptosis, is characterized by activation of caspases and scrambling of membrane phospholipids with externalization of phosphatidylserine (PS). Excessive eryptosis confers a procoagulant phenotype and is implicated in impairment of microcirculation and
Insulin resistance, ceramide accumulation, and endoplasmic reticulum stress in human chronic alcohol-related liver disease
Longato L, et al.
Oxidative Medicine and Cellular Longevity, 2012 (2012)
Role of sphingomyelinase in mitochondrial ceramide accumulation during reperfusion
Ramirez-Camacho I, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1862(10), 1955-1963 (2016)
The sphingolipid salvage pathway in ceramide metabolism and signaling
Kitatani K, et al.
Cellular Signalling, 20(6), 1010-1018 (2008)

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