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Documenti fondamentali

C-39310

Sigma-Aldrich

Fibroblast Growth Medium

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Sinonimo/i:

Recommended with C-23210

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.81

Stato

liquid

Confezionamento

pkg of 1 kit

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Fibroblast Growth Medium SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23210

Applicazioni

Fibroblast Growth Medium is a serum-free medium optimized for the cultivation of neonatal and juvenile fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), juvenile foreskin

Componenti

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualità

All lots of PromoCell Fibroblast Growth Medium are subjected to comprehensive quality control tests using primary human fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota sulla preparazione

Final supplement concentrations after addition to the basal medium:Basic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

Stoccaggio e stabilità

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Mantenimento delle subcolture

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Codice della classe di stoccaggio

10 - Combustible liquids


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Articoli

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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