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Key Documents

C-24215

Sigma-Aldrich

Melanocyte Growth Medium

Basal Medium, phenol red-free, 500 ml

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.81

Forma fisica

aqueous solution

Confezionamento

pkg of 500 ml

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Melanocyte Basal Medium, phenol red-free (supplements sold separately)

Applicazioni

Melanocyte Growth Medium is a low-serum (0.2%), PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M2 (C-24300). The Melanocyte Growth Medium is optimized for primary human cells, but can also be used for equine, porcine, murine, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin

Componenti

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualità

All lots of PromoCell Melanocyte Growth Medium are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Stoccaggio e stabilità

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Mantenimento delle subcolture

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.

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