C1985
Anti-Clathrin Light Chain antibody, Mouse monoclonal
clone CON.1, purified from hybridoma cell culture
Sinonimo/i:
Anti-LCB
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About This Item
Prodotti consigliati
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Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
CON.1, monoclonal
Forma fisica
buffered aqueous solution
PM
antigen 25-36 kDa (doublet)
Reattività contro le specie
human, bovine, mouse, yeast, rat
Concentrazione
~2 mg/mL
tecniche
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole rat brain homogenate
Isotipo
IgG2b
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... CLTA(1211) , CLTB(1212)
mouse ... Clta(12757) , Cltb(74325)
rat ... Clta(83800) , Cltb(116561)
Descrizione generale
Clathrin, light chain A also known as LCA is a soluble protein composed of heavy and light chain. Clathrin is a protein that plays a major role in the formation of coated vesicles. It coats vesicles from brain, adrenal medulla and non-secreting lymphoma cell line. Clathrin-coated vesicles (CCVs) are major carriers for endocytic cargo and maintain important intracellular trafficking events.
Immunogeno
synthetic peptide (amino acids 23-44 in bovine) common to the N-terminus of mammalian clathrin light chains, LCa and LCb, conjugated to KLH. This region is not involved in heavy chain binding and is completely conserved in all mammals.
Applicazioni
Monoclonal Anti-Clathrin Light Chain antibody produced in mouse is suitable for the following applications:
- Immunocytochemistry
- Immunoprecipitation
- Microarrary
- Western blotting at a concentration of 1-2μg/mL using whole rat brain homogenate
Azioni biochim/fisiol
Clathrin requires cofactors like ADP-ribosylation factor 6 (ARF6) and adaptin for regulation of receptors. Clathrin light chains (CLCs) are not required for clathrin-mediated endocytosis but plays a critical role for clathrin-mediated trafficking between the TGN and the endosomal system whereas, clathrin heavy chain provides the structural backbone of the clathrin coat. CLCs in mammalian cells function in intracellular membrane trafficking by acting as recruitment proteins for huntingtin-interacting protein 1-related (HIP1R), enabling it to regulate actin assembly on clathrin-coated structures.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Chen Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11790-11795 (2008-08-12)
During clathrin-mediated endocytosis, adaptor proteins recognize specific internalization signals on cargo receptors, either recruiting cargos into clathrin-coated pits (CCPs) or initiating clathrin-coat assembly around the cargo molecules. Here, we identify epsin 1, a clathrin-, ubiquitin-, and phospholipid-interacting protein, as a
Delia Bucher et al.
Nature communications, 9(1), 1109-1109 (2018-03-20)
Although essential for many cellular processes, the sequence of structural and molecular events during clathrin-mediated endocytosis remains elusive. While it was long believed that clathrin-coated pits grow with a constant curvature, it was recently suggested that clathrin first assembles to
B M Pearse
Proceedings of the National Academy of Sciences of the United States of America, 73(4), 1255-1259 (1976-04-01)
Coated vesicles have been purified from brain, adrenal medulla, and a nonsecreting lymphoma cell line. A single major protein species, clathrin, with an apparent molecular weight of 180,000, forms the coat of all these vesicles. Peptide mapping suggests that the
J A Ybe et al.
The EMBO journal, 17(5), 1297-1303 (1998-04-18)
Clathrin self-assembly into a polyhedral lattice mediates membrane protein sorting during endocytosis and organelle biogenesis. Lattice formation occurs spontaneously in vitro at low pH and, intracellularly, is triggered by adaptors at physiological pH. To begin to understand the cellular regulation
Daniel G Booth et al.
The EMBO journal, 30(5), 906-919 (2011-02-08)
Kinetochore fibres (K-fibres) of the spindle apparatus move chromosomes during mitosis. These fibres are discrete bundles of parallel microtubules (MTs) that are crosslinked by inter-MT 'bridges' that are thought to improve fibre stability during chromosomal movement. The identity of these
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