C-14037

Human Aortic Adventitial Fibroblasts (HAoAF)

1 million cells per pellet

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human aorta (adventitial tissue)
• Confezionamento: pkg of 1,000,000 cells
• Morfologia: (fibroblast)
• applicazioni: cell analysis (DNA, RNA, and protein)
• Malattie correlate: hypertension; cardiovascular diseases
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

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PromoCell Cell Pellets are stored in RNAlater- an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.

Note: RNAlater denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 μl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.

Origine della linea cellulare

Adventitial Tissue

Applicazioni

Primary Human Aortic Adventitial Fibroblasts (HAoAF) are isolated from human aortic adventitial tissue. HAoAF can be used for studies concerning vascular diseases e.g. neointimal formation, vascular remodeling, atherosclerosis, and hypertension. They are also often used for the examination of fibroblast disorders like fibrosis or other diseases linked to either imperfect or excessive accumulation of fibroblasts.

Qualità

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Stato fisico

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Mantenimento delle subcolture

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Altre note

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Note legali

RNAlater is a registered trademark of Ambion, Inc.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable