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C1374

Sigma-Aldrich

CDK6/CyclinD1, active, His/GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

CCND1, Cell division protein kinase 6, BCL1, CDK6, D11S287E, MGC59692, PLSTIRE, PRAD1, U21B31

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Ricombinante

expressed in baculovirus infected Sf9 cells

Livello qualitativo

200

Nome Commerciale

PRECISIO® Kinase

Saggio

≥70% (SDS-PAGE)

Stato

buffered aqueous glycerol solution

Attività specifica

7.9-10.7 nmol/min·mg

PM

CDK6 subunit ~40 kDa
cyclin D1 ~61 kDa

tecniche

activity assay: suitable

Solubilità

soluble
water: soluble

N° accesso UniProt

P24385
Q00534

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... CCND1(595) , CDK6(1021)

Descrizione generale

Research area: APOPTOSIS

This recombinant product was expressed by baculovirus in Sf9 insect cells using an N-terminal His-tag on CDK6, and a GST-tag on Cyclin D1.

Applicazioni

CDK6/CyclinD1, active, His/GST tagged human has been used in: In vitro CDK6 activity assay to evaluate the effects of small-molecule inhibitors for therapeutic haematopoietic stem cells (HSC) expansion.

Azioni biochim/fisiol

Cyclin Dependent Kinases6 (CDK6) is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. CDK6 kinase activity is detected during the mid-G1 phase of the cell cycle. It plays a crucial role in phosphorylating and regulating the activity of the retinoblastoma (RB), a tumor suppressor protein. Moreover, CDK6 has active involvement during transcription in hematopoietic stem cells (HSC) and tumor cells. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein. The activation of cyclin D1-dependent kinase activity is found to be necessary to trigger the process of apoptosis.

Stato fisico

Supplied in 50 mM Sodium Phosphate, pH 7.0, with 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, and 25% glycerol.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL8676
SLCF5339
SLBL8133V
SLBH8649

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Tohru Takaki et al.
Journal of biochemistry, 137(3), 381-386 (2005-04-06)
D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we
M Meyerson et al.
Molecular and cellular biology, 14(3), 2077-2086 (1994-03-01)
A family of vertebrate cdc2-related kinases has been identified, and these kinases are candidates for roles in cell cycle regulation. Here, we show that the human PLSTIRE gene product is a novel cyclin-dependent kinase, cdk6. The cdk6 kinase is associated
Haizhen Wang et al.
Nature, 546(7658), 426-430 (2017-06-14)
D-type cyclins (D1, D2 and D3) and their associated cyclin-dependent kinases (CDK4 and CDK6) are components of the core cell cycle machinery that drives cell proliferation. Inhibitors of CDK4 and CDK6 are currently being tested in clinical trials for patients
Yingdai Gao et al.
Nature communications, 6, 6328-6328 (2015-02-19)
Among cyclin-dependent kinase inhibitors that control the G1 phase in cell cycle, only p18 and p27 can negatively regulate haematopoietic stem cell (HSC) self-renewal. In this manuscript, we demonstrate that p18 protein is a more potent inhibitor of HSC self-renewal

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