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Merck
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Key Documents

C-12917

Sigma-Aldrich

Human M2 Macrophages (hMDM-MCSF(-))

Monocyte-derived, single donor, 5 million cryopreserved cells

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81

Origine biologica

human blood (monocyte)

Confezionamento

pkg of 500,000 cells

Morfologia

(macrophages)

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−196°C

Categorie correlate

Descrizione generale

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origine della linea cellulare

Monocyte

Applicazioni

Cryopreserved human monocyte-derived macrophages (hMDM) are convenient and easy-to-handle. The thawed cells plate into all tissue culture vessel formats and can be maintained as adherent, biologically functional cultures for several weeks. Optionally, user-customizable activation of the cells can be performed.Cryopreserved human macrophages are produced from monocytes in M2 Macrophage Generation Media DXF and are available as non-activated, fully qualified M2- (M-CSF) polarized cells. After 9-10 days of differentiation, the M2 macrophages are cryopreserved using Cryo-SFM. Each cryo vial contains more than 5 million viable cells after thawing.

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

Click here for more information.

Altre note

Recommended Plating Density: 200000 cells per cm2Tested Markers: CD163 positive, CD68 positiveNote: The plated cells do not proliferate. Recommended plating density for obtaining an adherent cell monolayer with 70-90% confluency.

Prodotti consigliati

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Protocolli

In Vitro Differentiation of Human PBMC Derived Monocytes into M1 or M2 Macrophages in a Serum-free and Xeno-free Cell Culture Media

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.