C-12221

Human Coronary Artery Endothelial Cells (HCAEC)

500,000 cryopreserved cells

• Sinonimo/i: HCAEC cells
• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: human coronary artery
• Confezionamento: pkg of 500,000 cells
• Morfologia: ( endothelial)
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

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Origine della linea cellulare

Artery

Applicazioni

Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor and are provided in a cryopreserved format. They are crucially involved in the regulation of coronary blood flow and cardiac functions and are consequently useful for in vitro studies of diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also suitable for studying functional vasodilators, such as prostacyclin, bradykinin, or nitric oxide.

Qualità

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

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Altre note

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P3Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable