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B4554

Sigma-Aldrich

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

Sinonimo/i:

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine

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About This Item

Formula empirica (notazione di Hill):
C6H15NO5S
Numero CAS:
10191-18-1
Peso molecolare:
213.25
Beilstein:
1781572
Numero CE:
233-465-5
Numero MDL:
MFCD00007533
Codice UNSPSC:
12161700
ID PubChem:
24891788
NACRES:
NA.25

Grado

BioPerformance Certified

Saggio

≥99.0%

Forma fisica

crystalline

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin and bioburden, tested

pH

2.5-5.0 (25 °C, 213.3 g/L)

Intervallo di pH utile

6.4-7.8

pKa (25 °C)

7.1

Solubilità

water: 0.5 g/mL, clear, colorless

applicazioni

diagnostic assay manufacturing

Attività estranea

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

Temperatura di conservazione

room temp

Stringa SMILE

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
AJTVSSFTXWNIRG-UHFFFAOYSA-N

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Descrizione generale

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Applicazioni

BES buffer has been used:

  • as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
  • as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
  • as a component of diluent one for semen collection and cryopreservation

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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