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B1267

Sigma-Aldrich

Biotin-maleimide

≥95% (TLC), powder

Sinonimo/i:

N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide

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About This Item

Formula empirica (notazione di Hill):
C20H29N5O5S
Numero CAS:
116919-18-7
Peso molecolare:
451.54
Codice UNSPSC:
12352203
ID PubChem:
329773179
NACRES:
NA.46

Livello qualitativo

200

Saggio

≥95% (TLC)

Stato

powder

Solubilità

acetic acid: 20 mg/mL

Temperatura di conservazione

2-8°C

Stringa SMILE

O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23

InChI

1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
VAPZWEFPQRDVHR-NJSLBKSFSA-N

Categorie correlate

Descrizione generale

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.

Applicazioni

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000278752
0000278752
SLCL4329
SLCD4596
SLCB0798

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Sigma-Aldrich

Sigma-Aldrich

S9415

Streptavidin−Maleimide from Streptomyces avidinii

N-[2-(Dansylamino)ethyl]maleimide BioReagent, suitable for fluorescence, ≥99.0% (HPLC)

Sigma-Aldrich

39326

N-[2-(Dansylamino)ethyl]maleimide

Maleimide 99%

Sigma-Aldrich

129585

Maleimide

Biotin-2′,2′,3′,3′-d4 ≥98 atom % D, ≥95% (CP)

Sigma-Aldrich

809608

Biotin-2′,2′,3′,3′-d4

K Gregorius et al.
Analytical biochemistry, 299(1), 84-91 (2001-12-01)
The orientation of an immobilized antigen is important for recognition by, e.g., an antibody. When noncovalent passive adsorption is used for immobilization, the number of ways that the antigen can attach to the surface is numerous and control of how
E A Bayer et al.
Analytical biochemistry, 149(2), 529-536 (1985-09-01)
A biotin-containing, thiol-specific reagent, 3-(N-maleimido-propionyl) biocytin (MPB), was synthesized and used to biotinylate various proteins via native or artificially induced sulfhydryl groups. In combination with appropriate avidin- or streptavidin-conjugated markers (i.e., fluorescent, enzyme-conjugated, electron-dense, etc.), MPB essentially constitutes a universal
Camilla To Benfield et al.
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Significant evidences indicate that reactive oxygen species (ROS) can induce macroautophagy/autophagy under both physiological and pathological conditions. Although the relationship between ROS and autophagy regulation has been well studied, the basic mechanism by which ROS affects autophagy and the biological

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