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ADD620C

Sigma-Aldrich

HepaRG Maintenance and Metabolism Medium Supplement with Antibiotics

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

Forma fisica

liquid

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Biopredic′s HepaRG Maintenance and Metabolism Medium Supplement with Antibiotics can be used with all HepaRG cell lines. See user guide for detailed protocols.
HepaRG cells uniquely maintain significant levels of hepatic cell functions, being CYP450 inducible and support the complete hepatitis B virus (HBV) replicative cycle.

Applicazioni

HepaRG maintenance and metabolism medium supplement with antibiotics has been used to culture HepaRG cells:
  • to study the interaction of hepatocytes and cholangiocytes when challenged with chlorpromazine (CPZ)
  • to assess the validity of stable reference genes (RG) for gene expression using quantitative real-time polymerase chain reaction (RT-qPCR)
  • as a supplement in basal hepatic cell culture medium to study the effects of cholestasis on liver connexins

Note legali

Use of this product is subject to one or more license agreements.

HepaRG cells are patented and their use is strictly limited; consider the cells as a single use, disposable product that must be destroyed upon conclusion of a study or experiment. Propagating, reproducing, cloning, subcloning or any other use of the cells following the conclusion of a study is prohibited. Use of the cells to produce or manufacture commercial products for general sale or for use in the manufacture of products intended for general sale is prohibited. Transfer of the cells to anyone not employed within the same organization, whether for financial benefit or not, is prohibited. If you are unwilling to accept the terms of this LIMITED USE LICENSE, do not ORDER or use them and immediately return the cells for credit. Violators of this Limited Use Licesense will be prosecuted to the fullest extent of the law.
HepaRG is a trademark of BioPredic International company

Pittogrammi

Health hazard

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Repr. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Beth Williamson et al.
Pharmacology research & perspectives, 4(5), e00264-e00264 (2016-10-08)
The nuclear pregnane X receptor (PXR) regulates the expression of genes involved in the metabolism, hepatobiliary disposition, and toxicity of drugs and endogenous compounds. PXR is a promiscuous nuclear hormone receptor (NHR) with significant ligand and DNA-binding crosstalk with the
Catherine C Bell et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(4), 419-429 (2017-02-01)
Reliable and versatile hepatic in vitro systems for the prediction of drug pharmacokinetics and toxicity are essential constituents of preclinical safety assessment pipelines for new medicines. Here, we compared three emerging cell systems-hepatocytes derived from induced pluripotent stem cells, HepaRG
Anika Mann et al.
Human cell, 30(4), 267-278 (2017-05-21)
HepaRG cells are widely used as an in vitro model to assess drug-induced hepatotoxicity. However, only few studies exist so far regarding their suitability to detect the effects of drugs requiring a preceding activation via the cytochrome P450 (CYP) system.
Yi Ni et al.
Methods in molecular biology (Clifton, N.J.), 1540, 15-25 (2016-12-16)
Investigations of virus-host interactions rely on suitable in vitro cell culture systems that efficiently support virus infection. Such systems should ideally provide conditions that resemble those of natural host cells, e.g., the cell-type specific signaling and metabolic pathways. For HBV
Maxime Demazeau et al.
International journal of pharmaceutics, 524(1-2), 268-278 (2017-04-04)
In this study, we evaluated cationic liposomes prepared from diether-NH

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