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A9205

Sigma-Aldrich

Albumina sierica bovina

30% in saline, fatty acid free, aseptically filled

Sinonimo/i:

Albumina, Soluzione di BSA

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About This Item

Numero CAS:
9048-46-8
Numero MDL:
MFCD00130384
Codice UNSPSC:
12352202
NACRES:
NA.27

Origine biologica

bovine

Livello qualitativo

200

Sterilità

aseptically filled

Saggio

28-32% protein basis (biuret)

Stato

solution

Purificato mediante

cold ethanol fractionation

non contiene

stabilizer or preservative

Origine

USA origin

Concentrazione

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

tecniche

ELISA: suitable
electrophoresis: suitable
western blot: suitable

Impurezze

≤0.01% free fatty acid

N° accesso UniProt

P02769

Attività estranea

BT virus, none detected
VSV virus, none detected

Temperatura di conservazione

2-8°C

Informazioni sul gene

bovine ... ALB(280717)

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Descrizione generale

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Applicazioni

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Azioni biochim/fisiol

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Nota sulla preparazione

Initial fractionation by cold alcohol precipitation

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCJ3171
SLCH0318
SLCC4115
SLCC1678
SLBV4868

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Sieroalbumina bovina

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Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1
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