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A8185

Sigma-Aldrich

Ala-Gln

BioReagent, suitable for cell culture, suitable for insect cell culture

Sinonimo/i:

Alanyl-glutamine, Glutamine-S

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About This Item

Formula empirica (notazione di Hill):
C8H15N3O4
Numero CAS:
39537-23-0
Peso molecolare:
217.22
Numero MDL:
MFCD00133046
Codice UNSPSC:
12352209
ID PubChem:
24891211
NACRES:
NA.75

Nome Commerciale

BioReagent

Livello qualitativo

300

Saggio

≥98% (HPLC)

Stato

powder

tecniche

cell culture | insect: suitable
cell culture | mammalian: suitable

Colore

white to off-white

Temperatura di conservazione

room temp

Stringa SMILE

NC(CC[C@H](NC([C@@H](N)C)=O)C(O)=O)=O

InChI

1S/C8H15N3O4/c1-4(9)7(13)11-5(8(14)15)2-3-6(10)12/h4-5H,2-3,9H2,1H3,(H2,10,12)(H,11,13)(H,14,15)/t4-,5-/m0/s1
HJCMDXDYPOUFDY-WHFBIAKZSA-N

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Categorie correlate

Applicazioni

Ala-Gln has been used:
  • as a supplement in NeuralQ™ medium
  • as a component in growth medium and incubation medium (IM) for retinal cell lines
  • for experimental treatment of retinal cell lines respectively

Azioni biochim/fisiol

Alanyl-glutamine (Ala-Gln) is a dipeptide that is used as a substitute for glutamine in mammalian cell culture media, because of the instability of glutamine in solution and the formation of ammonia as a breakdown product of glutamine in solution, which can have deleterious effects on cells in culture. Ala-Glu is stable to heat sterilization and is less ammonia genic than glutamine, which contributes to its advantages as a media component. Ala-Gln has been utilized to investigate polymorphonuclear leucocyte and myeloperoxidase activity in vitro.

Risultati analitici

This product is cell culture tested (0.5 mg/ml) and insect cell culture tested (0.2 mg/ml). It is appropriate for use in cell culture and insect cell culture applications at 2-10 mM.

Note legali

NeuralQ is a trademark of GlobalStem

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000285302
0000285302
SLCH9807
SLCK9733
SLCK8944

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Vinicius Fernandes Cruzat et al.
Nutrition (Burbank, Los Angeles County, Calif.), 25(4), 428-435 (2008-12-06)
We investigated the effect of supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine, both in the free form, on the plasma and tissue concentrations of glutamine, glutamate, and glutathione (GSH) in rats subjected to long-duration
A high-throughput assay for mRNA silencing in primary cortical neurons in vitro with oligonucleotide therapeutics
Alterman JF, et al.
Bio-protocol, 7(16) (2017)
The adaptation of BHK cells to a non-ammoniagenic glutamate-based culture medium
Christie A, et al.
Biotechnology and Bioengineering, 64(3), 298-309 (1999)
Vinicius Fernandes Cruzat et al.
Cell biochemistry and function, 28(1), 24-30 (2009-11-04)
In this study, we investigated the effect of the supplementation with the dipeptide L-alanyl-L-glutamine (DIP) and a solution containing L-glutamine and L-alanine on plasma levels markers of muscle damage and levels of pro-inflammatory cytokines and glutamine metabolism in rats submitted
Tony E Haynes et al.
Amino acids, 37(1), 131-142 (2009-02-04)
This study tested the hypothesis that L-glutamine (Gln) or L-alanyl-L-glutamine (Ala-Gln) prevents oxidant- or endotoxin-induced death of neonatal enterocytes. Enterocytes of neonatal pigs rapidly hydrolyzed Ala-Gln and utilized Gln. To determine whether Gln or Ala-Gln has a cytoprotective effect, IPEC-1
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