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Documenti fondamentali

A7104

Sigma-Aldrich

Anti-AtMPK6 antibody produced in rabbit

affinity isolated antibody, liquid

Sinonimo/i:

Anti-Arabidopsis thaliana MPK6

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.44

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Grado

for molecular biology

Clone

polyclonal

Forma fisica

liquid

PM

antigen 47 kDa

Reattività contro le specie

Arabidopsis thaliana

tecniche

western blot: 0.25-0.5 μg/mL using extract (cytosolic fraction) of Arabidopsis thaliana leaves

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Arabidopsis thaliana ... ATMPK6(818982)

Descrizione generale

In Arabidopsis thaliana, 20 genes (MPKs) encode putative plant MAPKs, which can be grouped into four or five classes according to sequence similarity. All plant MAPKs are classified into the plant extracellular-regulated kinase/ERK (PERK) subfamily.

Immunogeno

synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK6 (amino acids 384-395). This sequence is specific to AtMPK6 (not found in the other 20 known AtMPK isoforms). It shares limited homology (40-50% identity) to the AtMPK3 and AtMPK4 isoforms, and is highly conserved in the Nicotiana tabacum ortholog SIPK.

Applicazioni

Anti-AtMPK6 antibody produced in rabbit has been used in immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Azioni biochim/fisiol

Mitogen-activated protein kinase (MAPK) cascades are among the most conserved signal transduction pathways in eukaryotes. MAPKs regulate a variety of cellular activities ranging from gene expression, cell proliferation, motility, metabolism and apoptosis. In Arabidopsis thaliana putative functions have been assigned to AtMPK3, AtMPK4 and AtMPK6 and all three MAPKs have been linked to diverse stress responses, including oxidative stress signaling, pathogen defense, drought, high salt, cold and touch.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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A Suite of Receptor-Like Kinases and a Putative Mechano-Sensitive Channel Are Involved in Autoimmunity and Plasma Membrane-Based Defenses in Arabidopsis
Zhang Z, et al.
Molecular plant-microbe interactions : MPMI, 30(2), 150-160 (2017)
Emerging MAP kinase pathways in plant stress signalling
Nakagami H, et al.
Trends in Plant Science, 10(7), 339-346 (2005)
Agnieszka Ludwików et al.
Molecular plant, 7(6), 960-976 (2014-03-19)
Ethylene plays a crucial role in various biological processes and therefore its biosynthesis is strictly regulated by multiple mechanisms. Posttranslational regulation, which is pivotal in controlling ethylene biosynthesis, impacts 1-aminocyclopropane 1-carboxylate synthase (ACS) protein stability via the complex interplay of
Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases
MAP Kinases in Plant Signal Transduction (2012)
Charlotte H Hurst et al.
Plant physiology, 177(2), 522-531 (2018-04-25)
Receptor-like kinases (RLKs) are the largest family of proteins in plants and are responsible for perceiving the vast majority of extracellular stimuli. Thus, RLKs function in diverse processes, including sensing pathogen attacks, regulating symbiotic interactions, transducing hormone and peptide signals

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