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Key Documents

A5384

Sigma-Aldrich

Aconitase from porcine heart

Sinonimo/i:

Aconitate hydratase, Citrate (isocitrate) hydrolyase

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Porcine heart

Forma fisica

solid

PM

~66 kDa by gel filtration

Temperatura di conservazione

−20°C

Applicazioni

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

Azioni biochim/fisiol

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Definizione di unità

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Risultati analitici

Crude preparation containing aconitase activity

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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