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Documenti fondamentali

A2050

Sigma-Aldrich

Avidin–FITC from egg white

buffered aqueous solution

Sinonimo/i:

Avidin–Fluorescein isothiocyanate

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About This Item

Numero MDL:
MFCD00130585
Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

FITC conjugate

Stato

buffered aqueous solution

Grado di funzionalizzazione

3-5.5 mol fluorochrome per mol protein

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL
indirect immunofluorescence: 1:200

N° accesso UniProt

P02701

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

chicken ... AVD(396260)

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Descrizione generale

Avidin is homotetrameric protein (68 kDa) that is obtained from egg whites.

Applicazioni

Avidin-FITC from egg white has been used in:
  • flow cytometry
  • double immunostaining
  • double immunofluorescence
  • immunnohistochemistry

To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.

Stato fisico

Solution in 0.01 M phosphate buffered saline containing 10% (v/v) 0.5 M carbonate buffer, pH 9.5, and 15 mM sodium azide.

Nota sulla preparazione

Affinity purified protein.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
088M4794V
21180323
107M4881V
045M4830V
034M4758V

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Numerous cases of spinal cord injury following seizure have been previously reported. However, whether spinal cord injury is a common occurrence after seizures and its underlying mechanisms remain unclear. The present study generated a Sprague-Dawley rat model of temporal lobe
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8-Oxodeoxyguanosine is present in DNA from many tissues. The direct demonstration of 8-oxodeoxyguanosine as a potential biomarker of oxidative DNA damage has implications for the study of mutagenesis, carcinogenesis, and free radical toxicity. Avidin is shown here to bind with
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