A0412
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat
affinity isolated antibody
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About This Item
Prodotti consigliati
Origine biologica
goat
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Reattività contro le specie
mouse
tecniche
direct ELISA: 1:10,000 using IgG, IgA, IgM
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria, and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells,,. Goat anti-mouse polyvalent immunoglobulins (G,A,M)-peroxidase antibody binds to mouse IgG, IgA and IgM.
Applicazioni
The detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Relative binding affinities for specific Mabs were determined by an inhibition ELISA using peroxidase-conjugated goat anti-mouse polyvalent antibody diluted 1:10,000 in PBST and incubated for 1 hour at 21-23°C.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Nota sulla preparazione
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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We previously reported the enhanced resistance of monoclonal antibodies B6.1 (an immunoglobulin M [IgM]) and C3.1 (an IgG3) against experimental candidiasis. Both MAbs recognize the same fungal epitope. We have since found that a highly passaged B6.1 hybridoma (hp-B6.1) resulted
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