81349
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt
free from low molecular weight nucleotides, lyophilized
Sinonimo/i:
Poly(dI-dC) sodium salt
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About This Item
Prodotti consigliati
![Poly[d(I-C)] lyophilized, pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])](/deepweb/assets/sigmaaldrich/product/images/352/091/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1/640/ef743cea-ccd8-44f1-8f3b-dec5a1e4f5d1.jpg)
Grado
for molecular biology
Livello qualitativo
Stato
powder
Qualità
lyophilized
PM
Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000
Confezionamento
ampule of ≥10 units A260
Temperatura di conservazione
−20°C
Descrizione generale
Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.
Applicazioni
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.
Suitable for
- non-specific blocking agent in EMSA
- evaluation of DNA methyltransferases
- DNA-small molecule or drug interaction studies
Azioni biochim/fisiol
Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
Repeated probing of Southwestern blots using alkaline phosphatase stripping
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An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
L Skoog
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
Optimization of Competitor Poly (dI-dC) Poly (dI-dC) Levels is Advised in DNA-Protein Interaction Studies Involving Enriched Nuclear Proteins
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