79454

Ampliflu Red Westernblot Kit

for fluorescent detection

• Codice UNSPSC: 12352200

Proprietà

• impiego: sufficient for 50 applications
• Fluorescenza: λ_ex 571 nm; λ_em 585 nm
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

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Applicazioni

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

Principio

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

Risultati analitici

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

Note legali

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

I componenti del kit sono disponibili anche separatamente

• 90101 Ampliflu^™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)
• 79382 Phosphate buffered saline, tablet 5 tablets

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302
• Consigli di prudenza: P264 - P270 - P301 + P312 - P501
• Classi di pericolo: Acute Tox. 4 Oral
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable