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72464

Supelco

Atto 565 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Formula empirica (notazione di Hill):
C35H34ClN3O11
Peso molecolare:
708.11
Numero MDL:
MFCD03456131
Codice UNSPSC:
12352108
NACRES:
NA.32

Nome Commerciale

BioReagent

Livello qualitativo

100

Saggio

≥90% (HPLC)
≥90% (degree of coupling)

Forma fisica

powder

Produttore/marchio commerciale

ATTO-TEC GmbH

Compatibilità

suitable for fluorescence

Metodo di rivelazione

fluorometric

Temperatura di conservazione

−20°C

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Descrizione generale

Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.

Caratteristiche e vantaggi

  • Strong absorption
  • High fluorescence quantum yield
  • High thermal and photo-stability

Altre note

Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.

Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Elizabeth A Jares-Erijman et al.
Nature biotechnology, 21(11), 1387-1395 (2003-11-05)
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of
Benjamin Zoller et al.
Cell, 175(3), 835-847 (2018-10-20)
How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent transcriptional activity in early Drosophila embryos and characterize the variability in absolute activity levels across expression boundaries.
Michael Cooper et al.
Journal of fluorescence, 14(2), 145-150 (2004-12-24)
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an
Rachael Bakker et al.
eLife, 9 (2020-06-23)
Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the
Suman Lata et al.
Journal of the American Chemical Society, 128(7), 2365-2372 (2006-02-16)
Labeling of proteins with fluorescent dyes offers powerful means for monitoring protein interactions in vitro and in live cells. Only a few techniques for noncovalent fluorescence labeling with well-defined localization of the attached dye are currently available. Here, we present
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