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63689

Sigma-Aldrich

2-mercaptoetanolo

BioUltra, for molecular biology, ≥99.0% (GC)

Sinonimo/i:

β-mercaptoetanolo, 2-idrossietilmercaptano, BME, Thioethylene glycol

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About This Item

Formula condensata:
HSCH2CH2OH
Numero CAS:
60-24-2
Peso molecolare:
78.13
Beilstein:
773648
Numero CE:
200-464-6
Numero MDL:
MFCD00004890
Codice UNSPSC:
12161504
ID PubChem:
57651953
NACRES:
NA.25

Grado

for molecular biology

Livello qualitativo

200

Densità del vapore

2.69 (vs air)

Tensione di vapore

1 mmHg ( 20 °C)

Nome Commerciale

BioUltra

Saggio

≥99.0% (GC)

Stato

liquid

Limite di esplosione

18 %

Impiego in reazioni chimiche

reagent type: reductant

Concentrazione

14.3 M (pure liquid)

tecniche

RNA extraction: suitable
protein extraction: suitable

Impurezze

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

Indice di rifrazione

n20/D 1.500 (lit.)
n20/D 1.501

pH

4.5-6 (20 °C, 500 g/L)

P. ebollizione

157 °C (lit.)

Solubilità

H2O: 0.5 M at 20 °C, clear, colorless

Densità

1.114 g/mL at 25 °C (lit.)

Cationi in tracce

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.05 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

Stringa SMILE

OCCS

λ

0.5 M in H2O

Assorbanza UV

λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3

Temperatura di conservazione

2-8°C

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
DGVVWUTYPXICAM-UHFFFAOYSA-N

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Categorie correlate

Applicazioni

2-Mercaptoethanol has been used
  • in the protein extraction buffer prepared for leaf samples.
  • as a supplement in cell culture media.
  • in the procedure of RNA extraction.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

Avvertenze

Danger

Classi di pericolo

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Organi bersaglio

Liver,Heart

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

165.2 °F - closed cup

Punto d’infiammabilità (°C)

74 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCG5954
BCCG0639
BCCD5883
BCCC4884
BCCB7591

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Microfluidic extraction, stretching and analysis of human chromosomal DNA from single cells.
Benitez JJ et al.
Lab on a chip, 12, 4848-4848 (2012)
The interplay between protein L-isoaspartyl methyltransferase activity and insulin-like signaling to extend lifespan in Caenorhabditis elegans.
Khare S et al.
PLoS ONE, 6, e20850-e20850 (2011)
Adam Healey et al.
Plant methods, 10, 21-21 (2014-07-24)
Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within their leaf tissue making genomic DNA extraction difficult. While many DNA extraction
Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)
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Blood, 123(23), 3622-3634 (2014-04-24)
Previous studies demonstrated that imatinib mesylate (IM) induces autophagy in chronic myeloid leukemia (CML) and that this process is critical to cell survival upon therapy. However, it is not known if the autophagic process differs at basal levels between CML
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