Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

111M-1

Sigma-Aldrich

CD11c (5D11) Mouse Monoclonal Antibody

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

100
500

Coniugato

unconjugated

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

5D11, monoclonal

Descrizione

For In Vitro Diagnostic Use in Select Regions (See Chart)

Forma fisica

buffered aqueous solution

Reattività contro le specie

human

Confezionamento

vial of 0.1 mL concentrate (111M-14)
vial of 0.5 mL concentrate (111M-15)
bottle of 1.0 mL predilute (111M-17)
vial of 1.0 mL concentrate (111M-16)
bottle of 7.0 mL predilute (111M-18)

Produttore/marchio commerciale

Cell Marque

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20-1:80

Isotipo

IgG2a

Controllo

bone marrow, hairy cell leukemia

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Visualizzazione

cytoplasmic

Informazioni sul gene

human ... ITGAX(3687)

Categorie correlate

Descrizione generale

CD11c is an adhesion receptor of the leukocyte function-associated family of molecules. This cell surface antigen is normally expressed on granulocytes, monocytes, natural killer cells, and small populations of T- and B-lymphocytes. Therefore, the control for antibody validation and verification should be bone marrow and acute inflammatory tissue blocks. Anti-CD11c, clone 5D11, detects a formalin-resistant epitope of CD11c antigen and will now provide a significant change to the way hairy cell leukemia (HCL) can be diagnosed and assessed in FFPE. The tumor cells of the majority of other types of small B-cell lymphoma do not express this CD11c antigen. HCL is a distinctive yet uncommon chronic B-cell lymphoproliferative disorder, predominantly involving the bone marrow and spleen. Bone marrow aspiration and trephine biopsy are necessary for making a definitive diagnosis and treatment of hairy cell leukemia (HCL) although in exceptional cases, the diagnosis of HCL is made by histological analysis of splenic tissue after splenectomy or splenic needle core biopsy. However, aspiration is successful in only approximately 10% of patients. The marrow films obtained may demonstrate the presence of hairy cells but definitive diagnosis usually requires a bone marrow trephine biopsy due to the high frequency of a dry tap on aspiration. When there is difficulty in obtaining a good marrow aspirate, (frequent occurrence of a dry tap), examination of bone marrow trephine histology together with immunohistochemical analysis is the only available method to make a definitive diagnosis of HCL. Confirmation of the nature of the infiltrate can be obtained by immunocytochemistry performed on paraffin sections using antibodies against CD20, T bet, DBA.44 and TRAcP. However, expression of both TRAcP and DBA.44 (CD72) is not specific for HCL. It should also be noted, when undertaking immunohistochemistry to support a diagnosis of HCL in trephine biopsy sections, that up to 50% of cases show nuclear positivity for cyclin D1although confusion with mantle cell lymphoma (MCL) is less likely for an experienced hematopathologist as the cytology and histology of MCL are different from HCL. Korinna et al. in a recent study used clone (5D11) of anti-CD11c to investigate 31 bone marrow trephines with low-level HCL infiltrates and showed that the anti-CD11c was able to detect HCL to a level of 2% of tumor cells in BM biopsies. This indicates that immunohistochemical staining of formalin-fixed, decalcified bone marrow trephine biopsies with anti-CD11c can be used both for early diagnosis of HCL and for detection of residual disease following therapy. It is important to note that the CD11c-positive interstitial macrophages, which were generally more weakly stained than the hairy cells, did not interfere with the identification of the more strongly stained tumor cells. Among malignant lymphomas, CD11c is consistently expressed in HCL, although it is also rarely detected in B-CLL/small lymphocytic lymphoma, and nodal and extranodal marginal zone lymphoma (MZL).

Qualità


IVD

IVD

IVD

RUO

Stato fisico

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Nota sulla preparazione

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Altre note

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Note legali

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Non trovi il prodotto giusto?  

Prova il nostro Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Philip Th Went et al.
The American journal of surgical pathology, 29(4), 474-478 (2005-03-16)
Because of marrow fibrosis, bone marrow aspirations are often nonconclusive in patients with hairy cell leukemia (HCL). Therefore, histologic examination is important in HCL but often difficult in cases with low numbers of tumor cells. A combined immunohistochemical positivity for
R N Miranda et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 13(12), 1308-1314 (2001-01-06)
Mantle cell lymphoma (MCL) is more aggressive when compared with other lymphomas composed of small, mature B lymphocytes. Cyclin D1 is overexpressed in MCL as a result of the translocation t(11;14)(q13;q32). Cyclin D1 immunohistochemistry in fixed, paraffin-embedded tissue contributes to
Korinna Johrens et al.
Pathobiology : journal of immunopathology, molecular and cellular biology, 75(4), 252-256 (2008-06-27)
The diagnosis of hairy cell leukemia (HCL) and its distinction from other B-cell lymphomas can be difficult in formalin-fixed tissue. This is because the histology is not always classical, and despite having a characteristic phenotype, there are few relevant monoclonal
G Marotta et al.
Leukemia & lymphoma, 37(1-2), 145-149 (2000-03-18)
This study analyzed the expression of the beta2 integrin CD11c in 155 patients with well-characterized B-cell chronic lymphoproliferative disorders: 106 B-cell chronic lymphocytic leukemias (B-CLL), 21 hairy cell leukemias (HCL), 9 B-cell prolymphocytic leukemias (PLL) and 19 low grade non-Hodgkin's
Gail Jones et al.
British journal of haematology, 156(2), 186-195 (2011-11-25)
The British Committee for Standards in Haematology first produced guidelines for the diagnosis and management of hairy cell leukaemia and hairy cell leukaemia variant in 2000. This revision updates those guidelines and covers the areas of diagnosis, treatment and assessment

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.