09278

4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester

≥98.0% (HPLC)

• Sinonimo/i: 4-(Dimethylamino)azobenzene-4′-carboxylic acid N-succinimidyl ester, DABCYL-N-succinimidyl ester
• Formula empirica (notazione di Hill): C₁₉H₁₈N₄O₄
• Numero CAS: 146998-31-4
• Peso molecolare: 366.37
• Numero MDL: MFCD00467415
• Codice UNSPSC: 12352108
• ID PubChem: 329748752
• NACRES: NA.32

Proprietà

• Livello qualitativo: 100
• Saggio: ≥98.0% (HPLC)
• Stato: powder
• Solubilità: DMF: soluble; DMSO: soluble
• Fluorescenza: λ_ex 428 nm; λ_em 453 nm
• Temperatura di conservazione: −20°C
• Stringa SMILE: CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)C(=O)ON3C(=O)CCC3=O; CN(C)c1ccc(cc1)\N=N\c2ccc(cc2)C(=O)ON3C(=O)CCC3=O
• InChI: 1S/C19H18N4O4/c1-22(2)16-9-7-15(8-10-16)21-20-14-5-3-13(4-6-14)19(26)27-23-17(24)11-12-18(23)25/h3-10H,11-12H2,1-2H3/b21-20+; IBOVDNBDQHYNJI-QZQOTICOSA-N

Descrizione

Applicazioni

A useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies.

Risultati analitici

Absorption spectra shows a maximum at 453 nm (in methanol). After reaction with butylamine: max. absorption at 428 nm (Lit.)

Altre note

N-terminal modification of peptides in automated synthesis

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)