W0625

Wright stain

suitable for blood stain

• Sinonimo/i: Eosin Methylene blue according to Wright
• Numero CAS: 68988-92-1
• Numero MDL: MFCD00082143
• Codice UNSPSC: 12171500
• NACRES: NA.47

Proprietà

• Saggio: ≤4% (HPLC)
• Livello qualitativo: 200
• Forma fisica: powder
• Concentrazione: ≤4% (HPLC)
• Colore: dark green
• Solubilità: methanol: 10 mg/10 mL
• Compatibilità: suitable for blood stain
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1; AXIKDPDWFVPGOD-UHFFFAOYSA-O

Descrizione

Descrizione generale

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Applicazioni

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Caratteristiche e vantaggi

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Nota sulla preparazione

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H302 - H319
• Consigli di prudenza: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Classi di pericolo: Acute Tox. 4 Oral - Eye Irrit. 2
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves