PPB006

Tampone fosfato salino

powder, pH 7.4, pHast Pack^™

• Sinonimo/i: PBS
• Numero MDL: MFCD00131855
• Codice UNSPSC: 12161700
• NACRES: NA.21

Proprietà

• Nome del prodotto: Tampone fosfato salino, pH 7.4, pHast Pack^™, powder
• Nome Commerciale: pHast Pack^™
• Stato: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: <5—2 ppm Heavy metals (Lead)
• Colore: white
• pH: 7.4
• Solubilità: soluble, clear, colorless
• Cationi in tracce: Fe: <10 ppm
• Attività estranea: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Applicazioni

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Caratteristiche e vantaggi

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Confezionamento

Foil pouches

Ricostituzione

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable