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N2125

Sigma-Aldrich

Naphthol AS-BI phosphate

≥98% purity (HPLC), powder

Sinonimo/i:

7-Bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate

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About This Item

Formula empirica (notazione di Hill):
C18H15BrNO6P
Numero CAS:
Peso molecolare:
452.19
Beilstein:
6668526
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47

product name

Naphthol AS-BI phosphate, ≥98% (HPLC), Technical grade

Livello qualitativo

Saggio

≥98% (HPLC)

Forma fisica

powder

Colore

white to light yellow

Solubilità

methanol: 50 mg/mL

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

−20°C

Stringa SMILE

COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O

InChI

1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
HUXIAXQSTATULQ-UHFFFAOYSA-N

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Descrizione generale

Naphthol AS-BI, also known as 7-Bromo-3-hydroxy-2-naphtho-o-anisidine is a substituted naphthol.

Applicazioni

Naphthol AS-BI phosphate is suitable to use:
  • in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
  • as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
  • to perform alkaline phosphatase reaction
Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Azioni biochim/fisiol

Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Substrati

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


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The presence of a low molecular weight acid phosphatase in liver tissue that cannot be demonstrated with the histochemical substrate naphthol AS-BI phosphate.
A E Meijer et al.
Histochemistry, 67(1), 23-29 (1980-01-01)
Z Szegedi et al.
Pathology oncology research : POR, 4(3), 217-223 (1998-10-08)
The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis
W M Frederiks et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(2), 175-180 (1987-02-01)
Acid phosphatase activity has been demonstrated in rat liver with the semipermeable membrane technique using naphthol AS-BI phosphate as substrate and hexazotized pararosaniline (HPRA) as simultaneous coupling agent. With this method the final reaction product (FRP) appeared in rat liver
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
M Kimura et al.
Histochemistry, 82(6), 519-523 (1985-01-01)
A cytochemical study of acid phosphatase (AcP-ase) in the lateral prostate of the rat was performed to investigate whether AcP-ase in the secretory apparatus can be distinguished from AcP-ase in lysosomes and their related structures. Two types of AcP-ase were

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