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Key Documents

11815016001

Roche

Anti-HA Affinity Matrix

from rat IgG1

Sinonimo/i:

affinity matrix, anti-ha

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About This Item

Codice UNSPSC:
12352203

Origine biologica

rat

Livello qualitativo

Coniugato

agarose conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

3F10, monoclonal

Forma fisica

slurry

Confezionamento

pkg of 1 mL (settled resin volume)

Produttore/marchio commerciale

Roche

Isotipo

IgG1

Sequenza dell’epitopo

YPYDVPDYA

Capacità

2-8 nmol/mL binding capacity

Temperatura di conservazione

2-8°C

Descrizione generale

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

Specificità

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).

Immunogeno

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Applicazioni

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
  • Western blotting using the Anti-HA antibody
  • Silver staining (or similar protein stain)
  • In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
  • Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
  • Affinity column purification of HA-tagged proteins from crude protein extracts

Caratteristiche e vantaggi

  • Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
  • Use nondenaturing conditions for elution of your HA-tagged protein of choice.
  • Purify even rarely expressed HA-tagged proteins.
  • Highly specific to YPYDVPDYA.
  • Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
  • Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

Qualità

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

Stato fisico

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

Risultati analitici

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


Certificati d'analisi (COA)

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Protocolli

Anti-HA Affinity Matrix Protocol & Troubleshooting

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