11663925910

Mycoplasma PCR ELISA

• Codice UNSPSC: 12352200

Proprietà

• impiego: sufficient for 96 reactions
• Produttore/marchio commerciale: Roche
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Specificità

The Mycoplasma PCR ELISA is known to detect all of the following mycoplasma, acholeplasma, and ureaplasma species: M. orale, M. arginini, M. fermentans, M. hyorhinis, M. salivarium, M. gallisepticum, M. hominis, M. bovis, M. californicum, M. bovoculi, M. Pg50 bovine group, M. bovigenitalium, M. hyopneumoniae, A. laidlawii, U. urealyticum. DNA from other bacteria (except for Clostridium sp.), yeast, and eukaryotic cells are not detected.

Applicazioni

For research use only. Not for use in diagnostic procedures.
Mycoplasma PCR ELISA kit has been used for the detection of contamination by mycoplasma/acholeplasma/ureaplasma in cell cultures or other sample materials.

Mycoplasma PCR ELISA has been used to test mycoplasma contamination in cell lines.

Caratteristiche e vantaggi

Assay time: approximately 6 hours
Sample material: Cell culture supernatants, cell suspensions, or other samples
Sensitivity: At least 1 x 103 colony-forming units (cfu) of mycoplasma per milliliter of culture medium

• High sensitivity: Detects 1 - 10fg of DNA, corresponding to approximately 1 - 20 gene copies per reaction (at least 103cfu/ml)
• Broad detection range: All mycoplasma, acholeplasma, and ureaplasma species tested thus far were detected
• Simplicity: Detects all mycoplasma using the same protocol, as opposed to the culture method that requires different culture conditions for different mycoplasma species
• Fast results: The entire procedure can be performed in one day
• Easy handling of large numbers of samples: The ready-to-use mix and ELISA detection procedure allow fast and semi-automatic sample processing

Confezionamento

1 kit containing 14 components.

Sequenza

Chain Length Positive control: 520 bp.

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

• Lysis Reagent
• Neutralization Reagent
• PCR Mix ready-to-use
• Denaturation Reagent
• Hybridization Buffer
• Biotinylated Capture Probe
• Washing Buffer 10x concentrated
Anti-Digoxigenin-POD antibody
Conjugate Dilution Buffer
TMB Substrate Solution
Stop Reagent
Positive Control DNA
Streptavidin-coated Microplate
Adhesive Plate Cover Foils

Vedi tutto (14)

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H317,H319
• Consigli di prudenza: P261 - P264 - P280 - P333 + P313 - P337 + P313 - P362 + P364
• Classi di pericolo: Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash