11418165001
Roche
Biotin Protein Labeling Kit
sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA
Sinonimo/i:
Biotin, protein labeling
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About This Item
Codice UNSPSC:
12352200
impiego
sufficient for 5 labeling reactions
Livello qualitativo
Confezionamento
kit of 1 (5 components)
Produttore/marchio commerciale
Roche
tecniche
ELISA: suitable
immunoblotting: suitable
Temperatura di conservazione
2-8°C
Descrizione generale
For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.
Applicazioni
Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.
Confezionamento
1 kit containing 5 components.
Qualità
Function tested.
Nota sulla preparazione
Working solution: Preparation of the solutions
- Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
- PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
- Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).
Altre note
For life science research only. Not for use in diagnostic procedures.
Solo come componenti del kit
N° Catalogo
Descrizione
- Blocking Reagent (green screw cap)
- Phosphate Buffered Saline (PBS) (blue screw cap)
- D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
- Dimethylsulfoxide (DMSO) (red screw cap)
- Sephadex G-25 Columns
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
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