10108014001

Papain

from Carica papaya

• Sinonimo/i: cystein protease, papain
• Classificazione EC (Enzyme Commission): 3.4.22.2 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: papaya
• Livello qualitativo: 100
• Stato: suspension
• Attività specifica: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
• PM: 23 kDa
• Confezionamento: pkg of 10 mL (100 mg)
• Produttore/marchio commerciale: Roche
• Concentrazione: 10-100 mg/mL
• pH ottimale: 6.0-7.0
• Condizioni di spedizione: wet ice

Descrizione

Descrizione generale

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Applicazioni

Papain has been used in the digestion of various brain cells.

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Azioni biochim/fisiol

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Caratteristiche e vantaggi

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Stato fisico

Suspension, crystalline, nonsterile

Nota sulla preparazione

Working concentration: 0.05 to 0.5 mg/ml

Stoccaggio e stabilità

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash