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Key Documents

SCM018

Sigma-Aldrich

Embryoid Body (EB) Formation Medium

The Embryoid Body Formation Medium (EB Media) has been optimized & qualified to support the formation of embryoid body.

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Livello qualitativo

Forma fisica

liquid

tecniche

cell differentiation: suitable

input

sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

Condizioni di spedizione

dry ice

Descrizione generale

Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Most protocols used to differentiate murine ES cells to a variety of cells involve an initial step in which the spontaneous differentiation of ES cells proceed through the formation of embryoid body (EB). EBs are formed by culturing ES cells in suspension culture in low attachment Petri dishes. The non-adhesive surfaces of Petri dishes promote the aggregation of ES cells into a tight ball of cells termed embryoid body. EBs are thought to help maintain an embryonic organization and closely mimic some of the earliest stages of embryonic development.
The Embryoid Body (EB) Formation Medium has been optimized and qualified to support the formation of embryoid body. The medium can be used to form EB from hanging drops or suspension culture on non-adhesive Petri dishes. It has been validated for the differentiation of mouse ES cells into neural, adipogenic and cardiomyogenic cell lineages.

Stato fisico

Embryoid Body (EB) Formation Medium is a proprietary formulation that contains fetal bovine serum.

Stoccaggio e stabilità

Stored at -20°C until ready to use. Upon thawing, store at 2-8°C for up to one month.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

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Articoli

rganoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.