OP23

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

liquid, clone F235-1.7.1, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: F235-1.7.1, monoclonal
• Forma fisica: liquid
• contiene: ≤0.1% sodium azide as preservative
• Reattività contro le specie: rat, human, mouse
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: do not freeze
• Isotipo: IgG1
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... HRAS(3265)

Descrizione

Descrizione generale

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.

Recognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Immunogeno

Epitope: within amino acids 54-188

recombinant p21 Ras

Applicazioni

Frozen Sections (5 µg/ml)

Immunoblotting (10 µg/ml)

Immunofluorescence (2.5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Risultati analitici

Positive Control
Normal skin tissue or ras 1 cells

Altre note

For immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.

Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sidransky, D., et al. 1992. Science256, 102.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable