OP16
Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)
liquid, clone 7.16.4, Calbiochem®
Sinonimo/i:
Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.43
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
7.16.4, monoclonal
Forma fisica
liquid
contiene
≤0.1% sodium azide as preservative
Reattività contro le specie
rat, human
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
do not freeze
Isotipo
IgG2a
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... ERBB2(2064)
Descrizione generale
Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes ~185 kDa c-ErbB2 in overexpressing B104-1-1 cells. It is validated for WB, IP, ICC, frozen sections, & growth inhibition assay.
Purified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-ErbB2/c-neu protein.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
Immunogeno
B104-1-1 cells overexpressing rat c-ErbB2/c-Neu
Epitope: Within the extracellular domain
Human
Applicazioni
Frozen Sections (5 g/ml, see application references)
Immunoblotting (see application references)
Immunocytochemistry (5 g/ml)
Immunoprecipitation (1 g/ml, see application references)
Neutralization Studies (not recommended, see application references)
Confezionamento
Please refer to vial label for lot-specific concentration.
Attenzione
Toxicity: Standard Handling (A)
Stato fisico
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Risultati analitici
Negative Control
Fisher rat embryo cells
Fisher rat embryo cells
Positive Control
B104-1-1 cells
B104-1-1 cells
Altre note
Stains frozen tissue sections of rat cells overexpressing c-neu. Does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
Zhang, H., et al. 1999. Exp. Mol. Pathol.67, 15.
Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol.17, 1031.
Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA85, 5389.
Van De Vijver, M.J., et al. 1988. New Engl. J. Med.319, 1239.
DiFiore, P.P., et al. 1987. Science237, 178.
Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA84, 8498.
Slamon, D.J., et al. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Bargmann, C.I., et al. 1986. Nature319, 226.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory2, 215.
Peterson, N,C., and Greene, M.I., 1988. DNA Cell Biol.17, 1031.
Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA85, 5389.
Van De Vijver, M.J., et al. 1988. New Engl. J. Med.319, 1239.
DiFiore, P.P., et al. 1987. Science237, 178.
Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA84, 8498.
Slamon, D.J., et al. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Bargmann, C.I., et al. 1986. Nature319, 226.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory2, 215.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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