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Documenti fondamentali

MAMIC5S10

Millipore

Multiscreen® 96 well Plate, polycarbonate membrane

pore size 5.0 μm, sterile

Sinonimo/i:

MultiScreen PC Plate, Polycarbonate 96-Well Plate

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41104923
eCl@ss:
32039006
NACRES:
NB.22

Materiali

flat bottom wells
polycarbonate membrane
polystyrene

Livello qualitativo

Descrizione

Clear, Sterile, 5.0 µm pore size, Polycarbonate (PC) membrane, 10 plates

Sterilità

sterile

Nome Commerciale

MultiScreen®

Caratteristiche

lid

Produttore/marchio commerciale

MultiScreen®

Parametri

50-250 μL sample volume (per well)

tecniche

DNA purification: suitable

Superficie filtrante

0.28 cm2

Dimens. piastra

96 wells

Volume massimo del pozzetto

350 μL

Volume d’esercizio

50-75 μL

Dimensione pori

5.0 μm pore size

Tipo di binding

Tissue Culture (TC)-treated surface

Condizioni di spedizione

ambient

Descrizione generale

The MultiScreen®-MIC (Migration, Invasion, Chemotaxis) plate is a 96-well disposable device for use in functional assays for screening protein libraries and compound leads for drug discovery. It is designed to support high throughput cell-based functional assays such as migration, invasion, and chemotaxis with suspension and/or adherent cell lines, using a single device. The plates can be used to perform and quantify cell migration or invasion across coated or uncoated membranes in response to a concentration gradient. The MultiScreen-MIC plates are available in several pore sizes to accommodate MIC assays with a variety of cell lines and are intended for single use only.

Applicazioni

MultiScreen® Migration Invasion and Chemotaxis Filter Plate, 96 well has been used in the transwell/cellular migration assay using:
  • interleukin (IL)-4- and IL-12-treated T cells
  • THP-1 (human acute monocytic leukemia cell line)
  • in vitro activated, purified and IL-2-maintained galectin-3-deficient (gal3)-/- and gal3+/+ cluster of differentiation 4 (CD4)+ OTII T cells

Note legali

MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Huan-Yuan Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(34), 14496-14501 (2009-08-27)
We have investigated the function of endogenous galectin-3 in T cells. Galectin-3-deficient (gal3(-/-)) CD4(+) T cells secreted more IFN-gamma and IL-4 than gal3(+/+)CD4(+) T cells after T-cell receptor (TCR) engagement. Galectin-3 was recruited to the cytoplasmic side of the immunological
Jeremy Green et al.
Journal of medicinal chemistry, 58(12), 5028-5037 (2015-06-04)
The Rho kinases (ROCK1 and ROCK2) are highly homologous serine/threonine kinases that act on substrates associated with cellular motility, morphology, and contraction and are of therapeutic interest in diseases associated with cellular migration and contraction, such as hypertension, glaucoma, and

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