MABT880

Anti-SUN2 Antibody, clone 3.1E

clone 3.1E, from mouse

• Sinonimo/i: SUN domain-containing protein 2, Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 3.1E, monoclonal
• Reattività contro le specie: human, rat, mouse
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001186509.1
• N° accesso UniProt: Q9UH99
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SUN2(25777)

Descrizione

Descrizione generale

SUN domain-containing protein 2 (UniProt Q9UH99; also known as Protein unc-84 homolog B, Rab5-interacting protein, Rab5IP, Sad1/unc-84 protein-like 2) is encoded by the SUN2 (also known as FRIGG, KIAA0668, RAB5IP, UNC84B) gene (Gene ID 25777) in human. It is a single-pass nuclear envelope transmembrane protein that is highly expressed in heart, lung, and muscle. It has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. It may also facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage. It anchors chromosome movement in the prophase of meiosis and is required for telomere attachment to nuclear envelope and gametogenesis. SUN2 protein may also function on endocytic vesicles as a receptor for RAB5-GDP and participate in the activation of RAB5. Slight overexpression of SUN2 protein is seen in heart tissues form patients with congenital heart defects.

Specificità

Target specificity of clone 3.1E was verified by immunocytochemistry and Western blotting analyses using fibroblasts and heart tissue samples from Sun2-knockout mice.

Immunogeno

GST-tagged recombinant human SUN2 N-terminal LMNA-binding region fragment.

Applicazioni

Research Category
Cell Structure

This mouse monoclonal Anti-SUN2 Antibody, clone 3.1E, Cat. No. MABT880, is validated for use in Immunocytochemistry and Western Blotting for the detection of SUN2.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SUN2 in 10 µg of HepG2 cell lysate.

Immunocytochemistry Analysis: A representative lot immunostained nucleus of fibroblasts from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Western Blotting Analysis: A representative lot detected SUN2 in heart tissue lysate from Sun2+/-, but not Sun2-/- mice (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected mouse testis tissue (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot tested on different mouse tissues (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Immunofluorescence Analysis (IF): A representative lot detected immortalized fibroblasts derived from SUN2 +/+, SUN2 -/- , Sun1 -/- double knockout Sun2 -/- Sun1 -/- mouse pups (Courtesy of Dr Brian Burke, Institute of Medical Biology, A*STAR).

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SUN2 in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~80 kDa observed. 80.31/82.50/79.61 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1