MABT400
Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody
clone 5C11, from mouse
Sinonimo/i:
Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
5C11, monoclonal
Reattività contro le specie
canine, bovine, goat, rat, porcine, human, guinea pig
tecniche
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PRG4(10216)
Descrizione generale
The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.
Immunogeno
Recombinant protein corresponding to human Lubricin/Proteoglycan 4.
Applicazioni
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).
This Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.
Qualità
Evaluated by Western Blotting in Lubercin purified protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.
Descrizione del bersaglio
~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.
Stato fisico
Format: Purified
Altre note
Concentration: Please refer to lot specific datasheet.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Disulfide-bonded multimers of proteoglycan 4 PRG4 are present in normal synovial fluids.
Schmidt, TA; Plaas, AH; Sandy, JD
Biochimica et Biophysica Acta null
David C Browe et al.
Materials today. Bio, 16, 100343-100343 (2022-07-23)
Articular cartilage defects fail to heal spontaneously, typically progressing to osteoarthritis. Bone marrow stimulation techniques such as microfracture (MFX) are the current surgical standard of care; however MFX typically produces an inferior fibro-cartilaginous tissue which provides only temporary symptomatic relief.
Guido Carpino et al.
Clinical proteomics, 16, 37-37 (2019-11-07)
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