MABT38

Anti-Laminin subunit alpha-4 Antibody, clone 3H2

clone 3H2, from mouse

• Sinonimo/i: laminin, alpha 4, laminin subunit alpha-4, Laminin-8 subunit alpha, Laminin-9 subunit alpha, laminin alpha 4 chain, Laminin-14 subunit alpha
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 3H2, monoclonal
• Reattività contro le specie: human
• tecniche: ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_001098676
• N° accesso UniProt: Q16363
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... LAMA4(3910)

Descrizione

Descrizione generale

Laminins are basement membrane, extracellular matrix glycoproteins that are composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin subunit alpha-4 contains the C-terminal G domain, and although implicated in developmental regulation due to expression profiles, the biological function of laminin subunit alpha-4 is yet to be fully understood. Laminis as a whole play an important role in cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. High expression has been observed in adult heart, lung, ovary, small and large intestines, placenta, and liver, with lower levels observed in skeletal muscle, kidney, pancreas, testis, prostate, and rain.

Immunogeno

Recombinant protein corresponding to human Laminin subunit alpha-4.

Applicazioni

Anti-Laminin subunit alpha-4 Antibody, clone 3H2 detects level of Laminin subunit alpha-4 & has been published & validated for use in WB, IH, IP, ELISA.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Laminin subunit alpha-4 in basal cell carcinoma tissue.

ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Wondimu, Z., et al. (2004). Blood. 104(6):1859-1866.)

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualità

Evaluated by Western Blot in human serum tissue lysate under non-reduced conditions.

Western Blot Analysis: A 1:50,000 dilution of this antibody detected Laminin subunit alpha-4 in 10 µg of human serum tissue lysate (non-reduced conditions).

Descrizione del bersaglio

~202 kDa observed under non-reduced conditions.

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Human serum tissue lysate (non-reduced)

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable