MABT322

Anti-DDR2 Antibody, clone 2B12.1

clone 2B12.1, from mouse

• Sinonimo/i: Discoidin domain-containing receptor 2, CD167 antigen-like family member B, CD167b, Discoidin domain receptor 2, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TK
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 2B12.1, monoclonal
• Reattività contro le specie: human, mouse, rat
• tecniche: immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_001014796
• N° accesso UniProt: Q16832
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TKT(7086)

Descrizione

Descrizione generale

Discoidin domain-containing receptor 2 (EC 2.7.10.1; UniProt Q16832; also known as CD167 antigen-like family member B, CD167b, Discoidin domain receptor 2, Discoidin domain-containing receptor tyrosine kinase 2, Neurotrophic tyrosine kinase, receptor-related 3, Receptor protein-tyrosine kinase TKT, Tyrosine-protein kinase TYRO10) is encoded by the DDR2 (also known as NTRKR3, TKT, TYRO10) gene (Gene ID 4921) in human. Discoidin domain-containing receptors (DDRs) belong to the superfamily of transmembrane receptor tyrosine kinases (RTKs). DDRs are distinguished from other RTKs by their extracellular discoidin motif and their utilization of collagens as internal ligands. While DDR1 binds essentially all types of collagens, DDR2 shows a preference for type I, II and III fibrillar collagens, and nonfibrillar type X collagen. DDR1 is widely expressed in epithelial cells in lung, kidney, colon, and brain, whereas DDR2 is primarily expressed in mesenchymal cells including fibroblasts, myofibroblasts, smooth muscle cells, and chondrocytes in kidney, skin, lung, heart, and connective tissues. DDRs are important mediators of fundamental cellular processes, such as proliferation, survival, differentiation, adhesion and matrix remodeling, their dysregulations are reported to cause a number of human diseases, including fibrotic disorders, atherosclerosis and cancer. DDR2 is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature protein with an extracellular region (a.a. 22-399), followed by a transmembrane segment (a.a. 400-421) and a cytoplasmic region (a.a. 422-855) that harbors the kinase domain (a.a. 563-849).

Immunogeno

GST-tagged recombinant human DDR2 internal fragment.

Applicazioni

This Anti-DDR2 Antibody, clone 2B12.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DDR2.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of rat L6 myoblast and human HeLa cell lysates.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected DDR2 in human lung and kidney tissue sections.

Qualità

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DDR2 in 10 µg of NIH3T3 cell lysate.

Descrizione del bersaglio

~107/115 kDa observed. Target band(s) appears larger than the calculated molecular weight of 96.74 kDa due to glycosylation. A doublet banding pattern is often seen with the lower band representing less glycosylated DDR2 species.

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable