MABN1602

Anti-PIT-1 (POU1F1) Antibody, clone 8B6.1

clone 8B6.1, from mouse

• Sinonimo/i: Pituitary-specific positive transcription factor 1, GHF-1, Growth hormone factor 1, PIT-1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 8B6.1, monoclonal
• Reattività contro le specie: human
• tecniche: western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_000297
• N° accesso UniProt: P28069
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POU1F1 (5449)

Descrizione

Descrizione generale

Pituitary-specific positive transcription factor 1 (UniProt P28069; also known as GHF-1, Growth hormone factor 1, PIT-1) is encoded by the POU1F1 (also known as CPHD1, GHF1, PIT1) gene (Gene ID 5449) in human. PIT-1 is a transcription factor that serves as a master regulator of lineage commitment and expansion during the anterior pituitary development process that drives regional expansion and functional differentiation of five distinct hormone-producing cell lineages, including somatotropes, lactotropes, thyrotropes, corticotropes, and gonadotropes. Loss of Pit-1 expression results in a combined pituitary hormone deficiency (CPHD) syndrome reflecting the absence of lactotropes, somatotropes, and a subset of thyrotropes. Although originally characterized as pituitary-specific, PIT-1 is also expressed in human breast. PIT-1 upregulation in breast tumors increases cell proliferation and the expression of two breast cancer-related hormones, growth hormone (GH) and prolactin (PRL), leading to MMPs upregulation and breast cancer metastasis. Human POU1F1 gene encodes two alternatively spliced isoforms, where Pit-1A (GHF-2) a.a. 47-73 is truncated to a single Thr47 residue in Pit-1B (GHF-1). Both PIT-1 isoforms contain a PIT-1 POU-specific domain (a.a. 150-224 of Pit-1A or a.a. 124-198 of Pit-1B) and a DNA-binding Homeobox (a.a. 240-299 of Pit-1A or a.a. 214-273 of Pit-1B).

Specificità

Clone 8B6.1 epitope lies within an internal region present in both human PIT-1 spliced isoform A (Pit-1A, GHF-2) and isoform B (Pit-1B, GHF-1).

Immunogeno

GST-tagged recombinant human PIT-1 internal fragment.

Applicazioni

Anti-PIT-1 (POU1F1), clone 8B6.1 Antibody, Cat. No. MABN1602, is a highly specific mouse monoclonal antibody, that targets POU1F1 and has been tested in Western Blotting.

Qualità

Evaluated by Western Blotting in human pituitary tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIT-1/POU1F1 in 10 µg of human pituitary tissue lysate.

Descrizione del bersaglio

~33 kDa observed. 35.67 kDa (isoform A, Pit-1A, GHF-2) and 32.91 kDa (isoform B, Pit-1B, GHF-1) calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable