MABF3175

Anti-IL-5 Antibody, clone 4A1.6.15

• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 4A1.6.15, monoclonal
• PM: calculated mol wt 15.24 kDa; observed mol wt ~226 kDa
• Purificato mediante: using protein G
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 100 μg
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotipo: IgG2aκ
• Sequenza dell’epitopo: Unknown
• N° accesso ID proteina: NP_000870.1?
• N° accesso UniProt: P05113
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: mouse ... IL5(3567)

Descrizione

Descrizione generale

Interleukin-5 (UniProt: P05113; also known as IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF) is encoded by the IL5 gene (Gene ID: 3567) in human. IL-5 is a disulfide-linked homodimeric secreted protein that is synthesized and secreted mainly by the activated CD4+ helper T cells of the Th2 subset, mast cells, and eosinophils. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein (aa 20-134). It serves as a powerful proinflammatory cytokine that is responsible for maturation, proliferation, activation, and migration of eosinophils. It is also a key factor in the upregulation of myeloid progenitors in the bone marrow after airway allergen challenge, and for trafficking from the marrow to the airways in upper or lower airways inflammation. Its biological effects are mediated via selective interaction with IL-5 receptor (IL-5R), which is composed of an a subunit (IL-5Ra) and a non-specific βc heterodimer. As a homodimer it binds to IL-5Ra expressed on eosinophil surface and recruits the βc dimer and induces the assembly of the IL-5/IL-5Ra/βc ternary complex. In the absence of IL-5, IL-5Ra is complexed with the intracellular JAK2, whereas the βc subunit is associated with JAK1. (Ref.: Pelaia, C., et al. (2019). Front. Physiol. 10; 1514).

Specificità

Clone 4A1.6.15 is a mouse monoclonal antibody that detects human Interleukin-5 (IL-5).

Immunogeno

Full-length recombinant human Interleukin-5 (without the signal peptide).

Applicazioni

Quality Control Testing

Evaluated by Western Blotting with recombinant human IL-5.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant human IL-5.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-5 in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-5, clone 4A1.6.15, Cat. No. MABF3175, is a mouse monoclonal antibody that detects human Interleukin-5 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Stato fisico

Purified mouse monoclonal antibody IgG2a in PBS with 0.05% sodium azide.

Stoccaggio e stabilità

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable